Archiving cultures at −80 °C

Adapted from the lab compendium Snowflake Yeast Protocols — Ratcliff Lab / MuLTEE (V2).

Archiving for storage in −80 °C freezer

1. In a 1.5 mL tube, combine 750 μL culture with 750 μL 70% glycerol (sterile), for a final glycerol concentration of 35%.
2. Vortex at maximum speed (~3,000 rpm) briefly.
3. Label the tube (population, treatment, timepoint/transfer number, date) and transfer immediately to −80 °C.