MuLTEERatcliff Lab · Georgia Tech

Open Science / Phenotype data

Phenotype data

Phenotype measurements from the published MuLTEE papers, for each population, isolate and timepoint: cluster and cell size, cell shape, packing, budding, ploidy, mechanics, growth, fitness and more. Choose the traits to show, filter by treatment, line or timepoint, and click a value to see where it came from.

Phenotype measurements

Trait columns
Numbers show how many samples have a value in each column.

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All measurements (CSV)

One row per sample, one column per kind of measurement. A sample is a population, isolate or strain at one timepoint; its code gives treatment, line and timepoint (PA2_t600_iso1 is isolate 1 of line PA2 at transfer 600). Choose columns under Trait columns; each trait is split into columns that are comparable down the page (same statistic, similar growth time), and one-off measurements sit in that trait’s Other column.

Click any value (or hover over it) for how it was measured and where it was published. Values marked ≈ were read off a figure, so treat them as approximate. Values marked * were calculated from a paper’s data and are not reported in the paper itself. A dot means the sample was not measured for that column. Strains hides engineered strains by default; the categories are defined at the foot of this page.

Citing these data. Please cite the paper each value comes from.

Sample and strain codes match the Genome sequences table. To ask about measurements not listed here, see who to write to.


Trait guide

What each trait records

Cluster size
How large clusters grow, as a radius or diameter in µm. Size can be summarised in several ways: the mean radius, the biomass-weighted mean radius (which weights each cluster by its volume, so it follows where most of the biomass is), or the median. It also changes over the daily growth cycle.
Cell aspect ratio
Cell length divided by width, measured on individual cells released from clusters. More elongated cells pack less densely, which lets a cluster grow larger before it fractures.
Cell size
Cell volume in µm³, calculated from each cell’s long and short axes, or cell diameter where volume was not measured.
Ploidy
Genome copy number, in haploid genome equivalents (N). For isolates, the G1 peak of DNA content and the karyotype from genome sequencing. For populations, the main DNA-content peak and the fraction of cells below 3N, which shows how many diploid cells remain.
Macroscopic
Whether clusters grow large enough to see with the naked eye, around a millimetre across.
Entanglement
Whether branches from different parts of a cluster interlock, so the cluster stays in one piece even after the bonds between mother and daughter cells break.
Cell packing
The fraction of a cluster’s volume filled by cells. Denser packing builds strain sooner and limits how large a cluster grows before it breaks.
Budding pattern
Where and when cells divide: the angle at which daughters bud from their mother, how long the first and later divisions take, and whether daughters keep pace with their mothers.
Mechanical properties
How clusters respond to force: toughness, the stress at which they fracture, and the stiffness of individual cells.
Fluid flow
Whether metabolic activity drives fluid to flow through the inside of a cluster, carrying nutrients deeper than diffusion alone.
Growth
How fast biomass increases: cell doublings per daily transfer, or the doubling time of single cells.
Relative fitness
How well a strain competes against a labelled reference, usually as a selection rate per day under the experiment’s growth and settling selection.
Reproduction
How clusters produce new clusters, measured directly, for example as the increase in cluster number over one growth cycle.
Coexisting types
Whether distinct size types, Small and Large clusters, persist side by side in the same population.
Other
Strain-level measurements that fit none of the traits above, such as oxygen penetration, bud-scar size, chaperone levels, and the frequency of each size type within a population.

Strains

Strain categories

CategoryWhat it includes