Open Science
We are committed to open, transparent science, to collaboration, and to education. Strains, data, protocols and analysis code from the MuLTEE are all here.
What is available
Strains
Any of the fifteen populations from any archived timepoint, shared on request: how a request works, what the form collects, how to name the strains you want, and where to send it.
Genome sequences
Every public whole-genome sequencing run from the ancestor, timepoint isolates, population samples and derived strains, sortable by population, timepoint and paper, with direct links to the read files. The runs sit in four NCBI BioProjects, mainly PRJNA943273 and PRJNA1093477.
Phenotype data
Phenotype measurements from the published MuLTEE papers, by population, isolate and timepoint: cluster and cell size, cell shape and packing, budding pattern, ploidy, mechanics, growth, fitness and coexisting types. Click any value to see the paper and the figure, table or passage it came from.
Protocols
Daily culture and settling selection, archiving and revival, transformation, DNA and RNA extraction, and phenotyping (cluster size, cell shape, volume, ploidy).
Analysis pipelines
Kai Tong's ImageJ pipelines used in the protocols: cluster segmentation for measuring cluster size, and Cellpose-based cell segmentation for measuring cell size and shape.
Everything except strains can be downloaded without an account. For data not listed, see who to write to.
Contact
Who to write to
Dung T. Lac
Strain distribution, Ratcliff Lab
Send completed request forms here.
G. Ozan Bozdag
Principal investigator, NSF 2452109
Works out which populations and timepoints will answer your question, and what to expect from them.
William C. Ratcliff
Co-principal investigator, NSF 2452109
Collaborations, visits, and anything about the experiment as a whole.