Data & Strains
Access MuLTEE resources for your research
Request Strains
The Ratcliff laboratory freely shares strains from the MuLTEE frozen archive to advance scientific research. We ask researchers to complete a strain request form so we can ensure you receive the correct strains and avoid unintentional overlap with ongoing projects in the community.
Strain Request Process
To request strains, please download and complete the request form. The form collects:
- Recipient information for shipping and documentation
- Specific strains requested (strain names, timepoints, and treatments)
- Research plan so we can share relevant protocols and coordinate with other labs
- Shipping preferences (dry ice overnight vs. agar stabs)
Understanding your research goals helps us provide methodological support and ensures coordination across the growing community of labs studying multicellular evolution.
Download Strain Request Form (.docx)Submit completed forms to Dr. Ozan Bozdag at gonensin.bozdag@biology.gatech.edu
The Frozen Fossil Record
Every 25 days (approximately 125 generations), we cryogenically preserve all 15 evolving populations in a 35% glycerol solution at -80°C. This "frozen fossil record" now contains over 3,000 snapshots, allowing us to resurrect actual ancestors from any timepoint and test evolutionary hypotheses with modern technology. Strains are typically shipped within 2 weeks of approval.
Resources
Genome Sequences
Illumina short-read sequences for population samples and single-strain isolates from key timepoints.
Coming SoonPhenotype Data
Systematic measurements of cluster size, cell morphology, and fitness across the experiment.
Coming SoonProtocols
Detailed methods for working with snowflake yeast, including fitness assays and genetic transformation.
protocols.ioContact
For questions about strains, data, or collaboration opportunities, contact:
Dr. Ozan Bozdag
gonensin.bozdag@biology.gatech.edu
Dr. William Ratcliff
ratcliff@gatech.edu